Abstract: Histochemical studies were conducted on the anther development of two male-sterile lines and their fertile line in radish.The histochemical changes of male-sterile lines concided with the morphological changes.The inhibition of 475A anther development occurred before the stage of archesporial cell.At that time,the anther possessed little protein and nucleic acids, though some starch grains were observed.With the growth of anther, no protein,nucleic acids and starch grains were observed.Before the tetrad stage,the Spring Red Awas normal and stored rich polysaccharides,protein and nucleic acids.At the stage of nuinucleate microspore,the tapetal cells developed abnormally.During the abortion of pollen,the protein,polysaccarides and nucleic acids disappeared rapidly,and no starch grains were observed.