Abstract: Mulinzi nature sanctrary is located in the northeast portion of Mt. Wulingshan. Its natural geographic elements are superior. The saturation point of species is higher A total of 1043 species(including subspecies and varieties, the same below) is collected in the small area (2100 hectares) representing 488 genera and 134 families. Understanded deeply the quality, origin, relationship with neighbour flora of Mulinzi flora, the natural spermatophyte flora of 11 representative areas chosen in this paper are compared with Mulinzi flora. In comparative studies of a lot of flora, determining the dominant families and calculating the similarity index are the important parameter of indicating similarities and differences of flora. The similarity ind dexes of dominant families of spermatophyte flora of Mt. Mulinzi in compare with Mt Shenlongjia of Hubei Province, Mt. Lushan of Jiangxi Province, Mt. Heishiding of Guangdong Province and Mr. Jianfengling of Hainan Province are separately 73.3%, 46.7%, 33.3%, and 13.3%. The common dominant families having a lot of species under their command possess obvious substitute phenomenon in each flora. The genera of each spermatophyte flora are compared and analysed by the classical classification of types of distributional areas and similarity index. In addition, 14 types of distributional areas of 11 spermatophyte flora carry on agglomerative classification. The results are resemble to the results of classical flora study. It is fully proved that the methods of quantitative classification can objectively reflect the similarities and flora with other flora and their identity of origin.