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圆果黄麻雌性不育突变体鉴定及其生理生化特性初步研究

Identification of Jute(Corchorus capsularis L.) Female Sterile Mutant and Its Physio-biochemical Characteristics

  • 摘要: 为研究圆果黄麻(Corchorus capsularis L.)粤圆6号不育突变体的类型及不育机理,以粤圆6号及其不育突变体为实验材料,对其花器官及花粉粒进行了形态观察、碘染实验、体外萌发实验及田间授粉实验,结果表明,粤圆6号突变体为雌性不育。对两个材料发育不同时期功能叶和花蕾中的可溶性糖和可溶性蛋白含量进行了测定,结果表明,可溶性糖和可溶性蛋白在不同时期均表现出动态变化。雌性不育突变体功能叶中的可溶性糖在现蕾前期的含量略低于正常可育材料,进入现蕾期和结果期,其可溶性糖却比可育材料分别高出16.0%、33.3%,而花蕾组织中的可溶性糖比可育材料降低了15.5%;可育材料3个时期的功能叶及花蕾组织中可溶性蛋白的表达量分别比不育突变体高出3.9%、29.2%、79.1%、11.9%。上述代谢表达差异可能与雌性不育有关。

     

    Abstract: To study the jute’Yue Yuan 6’sterile mutant types and its sterile mechanism,morphological observation of pollen grain,iodine-dye assay,sprouting of pollen assay in vitro and pollination field experiments were carried out.The ’Yue Yuan 6’sterile mutants were female-sterile type.Soluble sugar and the soluble protein displayed dynamic change differently along with the growth time in functional leaves and flowers between the sterile mutant materials and the fertile materials.The content of soluble sugars in the sterile mutant material were lower than the fertile material in bud previous stage in functional leaves;however,this was opposite in the bud phase and productive phase(16.0% and 33.3%,respectively).Content of soluble sugar was lower in bud tissue of sterile mutant material than the fertile materials(15.5%).Soluble protein expression quantity in functional leaves and bud tissues of the fertile material were higher compared to the sterile mutant material in three different periods(3.9%,29.2%,79.1%,11.9%,respectively).The expression of metabolic difference of the above-mentioned may be related to female sterility.

     

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