根癌农杆菌对单子叶植物竹子的细胞转化

THE TRANSFORMATION OF MONOCOT-BAMBUSA CELLS WITH A. TUMEFACIENS

摘要: 本实验证明:具有重要经济价值的禾本科植物——竹子(bamboo)可被根癌农杆菌转化。在经有毒性的根癌农杆菌C₅₈、A₃₄₈感染的竹子材料中,分别测到了专性的胭脂碱和章鱼碱,而对照处理及无毒性的根癌农杆菌VirA₂₁₁^-mx、NTI感染的竹子材料中,测不到冠瘿碱。

Abstract: In this paper, the evidence is presented that a kind of commercial bamboo in Gramineae plant is subjected to Agrobacterium tumefaciens directly transformation. Strain specific nopaline and octopine have been detected respectively in bamboo cultures infected by A. tumefaciens C₅₈ and A₃₄₈ strains; while, opines are not observed when detect control tissues and the materials inoculated with NTI strains and VirA₂₁₁^- mx lost the virulence.