Abstract: The present paper deals with a study of the karyotype analysis of 5 species of genus Picea.The chromosome count and karyotypes of 4 species are reported for the first time,the numbers of chromosome of root tip cell have been found to be 24.3 species with B-chromosomes. The karyotype formula are as follows:P. koraiensis, 2n=24=20m(4sc)+4sm P. meyeri, 2n=24=22m(6sc)+2sm+2B P. wilsonii, 2n=24=16m(6sc)+8sm+2B P. schrenkiana, 2n=24=20m(6sc)+4sm P. jezonensis var. microsperma, 2n=24=22m(4sc)+2sm+1B.