Effects of Different Medium on the Growth and Photosynthetic Activity of Nostoc flagelliforme
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摘要: 研究测定了发菜(Nostoc flagelliforme Born.etFlah.)细胞在不同培养基中的生长速率、光合作用和叶绿素荧光活性。结果显示培养11d后:Detmer培养基中叶绿素a的含量为1.08mg/L,Kratz-Myers培养基中叶绿素a的含量为1.87mg/L,水生104号培养基中叶绿素a的含量为1.21mg/L,BG11培养基中叶绿素a的含量为2.18mg/L,表明在BG11培养基中培养的细胞具有最高生长速率;与另外4种不同浓度的BG11培养基相比,上述BG11培养基培养的发菜具有最大的光合速率Pm(218.1μmolO2·mg-1chla·h-1)和最高的PSII光化学效率(Fv/Fm=0.349)。实验结果表明,BG11是适合发菜生长的培养基,对其光合作用和叶绿素荧光活性具有显著促进作用。Abstract: The growth rate, photosynthesis and chlorophyll fluorescence of Nostoc flagelliforme Born.et Flah. cultured under different medium were tested. The results showed that after (11 d) cultivation, the content of chlorophyll a in different medium were (1.08 mg/L) (Detmer), (1.87 mg/L) (Kratz-Myers), 1.21 mg/L (hydrophilous 104), and 2.18 mg/L (BG11), respectively. N.flagelliforme in BG11 had the fastest growth rate. Compared with other concentrations of BG11, the N.flagelliforme cultured in BG11 had the highest gross photosynthesis Pm ((218.1 μmol) O2·mg-1(chl a)·h-1) and the highest ratio of chlorophyll fluorescence (Fv/Fm=0.349). The results indicated that BG11 is optimal to cultivate N.flagelliforme and improves its activities of photosynthesis and chlorophyll fluorescence markedly.
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Keywords:
- Nostoc flagelliforme /
- Chlorophyll a /
- Growth /
- Photosynthesis /
- Chlorophyll fluorescence
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