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猕猴桃胚乳培养中的胚胎发生

EMBRYOGENESIS IN ENDOSPERM CULTURE OF CHINESE GOOSEBERRY

  • 摘要: 中华猕猴桃及硬毛猕猴桃的胚乳培养在MS+Zeatin 3 ppm+2,4-D0.5ppm+CH 400ppm的培养基上诱导产生愈伤组织,在MS+Zeatin 1 ppm+CH 400ppm的分化培养基上产生胚状体和长成完整小植株。细胞组织学观察表明,胚状体起源于愈伤组织内单个细胞,经原胚、球形胚、心形胚等阶段发育成苗。猕猴桃胚乳属于细胞型胚乳,它的这种胚状体起源于愈伤组织内单个细胞的发生方式,也不同于常见的胚状体发生于愈伤组织表面的方式。

     

    Abstract: Endosperm of Actinidia chinensis var. chinensis and A. chinensis var. hispida were used as experimental materials. Exised endosperms were planted on mediumMS+Zeatin3 ppm+2, 4-D 0.5ppm+CH 400 ppm, white, friable and loose calluses were induced from endosperms. When the calluses were transferred on MS medium supplemented with Ⅰ ppm Zeatin and 400ppm CH, embryoids were produced about two weeks after inoculation, then normal plantlets with radicles and plumules emerged.Histocytological observations on embryogenesis showed that the embryoid in endosperm callus was originated from individual cell in callus. When calluses were transferred into differentiation medium, some cells in calluses were initiated and formed proembryoids and plantlets were further developed via globular embryoids and heart-shaped embryoids et al.The endosperm of Chinese gooseberry belongs to cellular type and the embryoid derived from individual cell, inside the callus. This way of origination is different from the usual way of initiation which is originated from the surface of callus.

     

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