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水体浊度对苦草光合荧光特性的影响

The Influences of Suspended Sands on Photosynthetic Fluorescence Characteristics of Vallisneria natans in Turbidity Waters

  • 摘要: 用粒径小于100μm的泥沙分别配置浊度为30、60、90 NTU(nephelometric turbidity units)的混浊水体,将苦草(Vallisneria natans)成株分别种植于上述水体中,利用水下饱和脉冲荧光仪(DIVING-PAM)测定植株最长叶片水下10 cm处ΔFv/Fm的光合日变化及快速光响应曲线(RLC)。结果表明,1个月后,水体浊度对苦草成体植株Fv/Fm和光合系统Ⅱ(PSⅡ)无显著影响,叶片ΔFv/Fm的日变化曲线呈“V”形;泥沙浊度≥60 NTU时,ΔFv/Fm降低幅度较小,光强降低时恢复较快,植株受光抑制的程度变小。快速光响应曲线结果显示,实验期间苦草开始衰老,光响应能力下降;随水体混浊度的升高,叶片光响应能力下降减慢。表明在浅(水深1.0 m)而浑浊的水体中,短时间内混浊水体对苦草成株叶片PSⅡ的影响较小。

     

    Abstract: Mature Vallisneria natans were planted in 30 NTU,60 NTU and 90 NTU turbid waters made by suspend sands with less than 100 μm,respectively.After one month,the longest leaf under 10 cm water were measured by a developed,submersible,pulse-amplitude modulated (PAM) fluorometer,and diving-PAM,and diurnal variation of ΔFv/Fm and rapid-light curves(RLC) were also measured.The results indicated that the turbid water had not significant influence on Fv/Fm,which indicated that photosystem II of V.natans hadn’t been influenced apparently.The diurnal variation of V.natans’s ΔFv/Fm in irradiance looked like "V" shape.When turbidity of water was more than 60 NTU,ΔFv/Fm of these plants decreased slightly,and restored quickly as irradiance became small.The results of RLCs suggested that V.natans began caducity,and their light responding ability of leaves decreased during the experimental period.With turbidity of water increasing,light responding ability of their leaves decreased slowly.All results indicated that the water turbidity had little influence on photosystem Ⅱ of these plants during the experiment.

     

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