Abstract:
The high molecular weight glutenin subunit gene,
1Ax1,is one of the determinant factors of processing properties,increasing the amount and composition can improve grain quality.In order to study the inheritance and stability of foreign
1Ax1 gene in wheat varieties,the crosses between transgenic wheat lines and elite wheat varieties have been carried out.Transgenic wheat line is(’B102-1-2’) in which the
1Ax1 gene has expressed and the two elite varieties are ’Chuan 89-107’ and(’Emai 12’) with high yield and stress-resistance.The parents,F
1 and F
2 generations from two crosses,with male parent being transgenic wheat,were tested for HMW-GS compositions by SDS-PAGE analysis,so as to explore the heredity of the foreign
1Ax1 in transgenic wheat line ’B102-1-2’,generated by particle bombardment.The results showed that the foreign
1Ax1 has been integrated into the target wheat varieties and expressed stably abi-(ding) by the Mendelian genetic law,which would be beneficial to wheat breeding program.