Abstract: It is the first time to describe the induction of calli, regeneration of plantlets and bulbs from Iphigenia indica Knuth—the plant containing colchicine (particularly from the organ containing colchicine). It may offer a way for rescuing this extincting plant.We found that in the weak light calli could be induced, in the strong light plantles regenerated from calli. NAA prevented growth and differentiation of calli. The pH 5.8 and pH 5.4 were correspondingly suitable for induction of calli and regeneration of plantlets.