RAPD双引物在构建红麻连锁图谱中的应用研究

详细信息

Applied Research on Construction of a Genetic Map of Kenaf Using Two-primer RAPD Combinations

Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops(Fujian Agriculture and Forestry University), Fuzhou 350002, China

摘要: 以阿联红麻(Alian Kenaf)与福红992(Fuhong992)杂交产生的F2代作图群体为研究材料,分别应用RAPD单引物和双引物进行多态性条带扩增,并进行扩增效果的比较研究,以期为作图群体构建红麻遗传连锁图谱奠定基础。结果表明,RAPD双引物比单引物扩增出更多的多态性条带,提高了引物的利用率和多态性条带的扩增效率。
- RAPD双引物 /
- 红麻 /
- 遗传连锁图谱
Abstract: RAPD and two-primer RAPD combinations were used to screen and compare for polymorphisms between Alain and Fuhong992,providing the foundation for constructing a genetic map of kenaf.The results showed that RAPD was a potential tool for constructing a genetic linkage map of kenaf.PCR reactions with two different primers tended to produce different amplification products than the single primer,so using two different primers was a convenient and economical way of improving polymorphism.
- Two-primer RAPD combinations /
- Kenaf /
- Genetic linkage map