Abstract: The flavonoids in the leaves of Sinocalycanthus chinensis from different populations and those in the different organs from the same population were extracted and separated by using polyamide TLC-chromatography.Every component was quantitatively analyzed by dual wavelength flying scanning using rutin as the standard sample.The results showed as the following.The content of flavonoids in Jiwoping population was the highest,while that in Zhongping population the second and that in Longtangshang population the lowest.There was significant difference among pair-wise populations,except for that between Shuangshibian population and Baishuiwu population.In the same population,the content of flavonoids in different organs was different.The content of flavonoids in the leaves was significantly higher than those in other organs.There was no significant difference among stems,one year twigs and two years twigs.Based on the analysis of TLC chromatography,seven components were found in the flavonoids extracted from the leaves except that there were only 5 components found in the flavonoids extracted from the leaves in Damingshan population.Six components were found in the flavonoids extracted from the roots,stems,one year twigs and two years twigs.The component with R_f equal to 0.66 was absent in leaves.