Abstract: The leaves from aseptuly plantets of Populus deltoids(I-63×I-69) were cultured as explants on 1/2(N)MS medium containing various combination of auxin and cytokinin.The results showed that the best medium for buds differentiation directly is 1/2(N)MS+0.2 mg/L BA+0.02 mg/L NAA+25 g/L sucrose+7 g/L agar,with the bud differentiation rate of 85%. In the experiments we also found KT has the stimulation to the plants rooting of I-63×I-69,the better rooting medium is MS+0.08 mg/L KT+(0.02 mg/L) NAA+25 g/L sucrose+7 g/L agar,with the rooting rate of 82.2%.