Abstract: The effects of different culture medium on the germination rate and time of Bletilla striata Rchb.f.(Orchidaceae) seeds in different developmental phases,and multiplication of cluster shoots and rootage of plantlets was investigated.The results showed that the germination rate was positively correlated with the embryo age and the embryo rate of seeds,whereas the germination time was passively correlated with them.For the seeds with 16-week embryo age,the germination rate could be reach 84% on the medium added with 1 g/L Huabao-1 and 2 g/L Huabao-2.The germination rate of the seeds with embryo age more than 20 weeks wasn’t affected by the culture medium element and could reach 100%.Moreover,its germination time only need 7 days.The best culture medium for multiplication of cluster shoots is 1/2 MS+4.0 mg/L 6-BA +0.2 mg/L NAA +100 g/L CM(coconut milk),on which the multiplication rate was 4.41.The medium with 1/2 MS+0.2 mg/L NAA was favorable to the growth of roots and the rate of rootage was 90%.