Abstract: The effects of different culture media,preservation condition and time on the germination of pollens collected from 30-40 years old Platanus acerifolia tree were studied.The result showed that the pollen germinating percentage reached the highest after incubated for (24 h) on the media supplemented with 15% sucrose and 0.01% Boric acid;solid medium supplemented with agar or 50 mg/L gibberelin(GA₃) wielded little influence over pollen germination;the dry pollen preserved under the temperature of 4℃ retained the viability 35 days and 20 days longer than the fresh pollen preserved under the temperature of 25℃ and 4℃,respectively.