Abstract: The function of a cold response regulator gene TBCBF3 on membrane composition was studied. Plasma membrane from transgenic plant Arabidopsis Columbia constitutively expressing CBF3 gene and from control was extracted, membrane lipids and proteins were separated for further analysis. The results show that total lipids, total proteins and phospholipids of plasma membrane increased in constitutively expressing CBF3 transgenic plant by 227%, 190% for totoal lipids, and total proteins, by 105.05% for phophoslipids. This effect is similar to that of cold acclimation, indicates that CBF3 induce the changes of the components in plasma membrane during cold acclimation, and play an important role in this process.