Abstract: The quantitative analysing method of luteoloside, demethoxyhaleniaside and haleniaside in Halenia ellipitica D.Don was set up by RP-HPLC, and this can provide scientific basis for cultivated halenia to replace wild halenia use as medicine. The standard curves of luteoloside,demethoxyhaleniaside and haleniaside showed good linearity over the range of 0.68-3.40 μg(r=0.9998),0.36-1.80 μg( (r=0.9990)and 0.80-4.00 μg(r=0.9979),respectively. The average recovery was 100.39% (RSD=2.60%), 99.79% (RSD=3.55%) and 100.71%(RSD=2.19%), respectively. The content of haleniaside and demethoxyhaleniaside (compounds of anti-hepatitis) has no obvious difference between in wild and in cultivated halenia, and get the preliminary testimony that cultivated speciecan replace wild specie to use as medicine.