Abstract: A procedure had been developed for cryopreservation of Glycyrrhiza inflata Batal suspension cultures by vitrification.High flavonoid-yield G.inflata Batal suspension cultures are valuable materials but hard to be preserved.The key point of this research is how to reduce the damage of cell caused by the sharp change of pressure.So setting up a suitable method must depend on various important factors such as the duration of regrowth,the harvest time,the duration of dehydration,the sucrose concentration of washing solution,etc.Survival rate of G.inflata Batal suspension culture cell was 82.9% by TTC examination,and regeneration rate reached to 80.4%.