Abstract:
The effects of different hormone on axinary buds induction,tufty bud differentiation,and rooting were studied by means of orthogonal design and common buckwheat(
Fagopyrum esculentum Moench)stem segment explants with a knot. The results showed that 6-BA is a major factor for induction of axianry buds and roots,and that TDZ is the key to induction of tufty bud.The optimal medium for inducing axillary bud development is MS
0+6-BA 1.0 mg/L+NAA 0.1 mg/L,with the rate of axillary bud development of 67.9%.Among them,the second and third knot can produce the rate of 80% or more.The MS
0 medium containing 6-BA 4.0 mg/L+TDZ 0.06 mg/L is better for inducing tufty buds with the differentiation rate of 166.7%.The optimum medium of root formation is 1/2 MS
0+6-BA 1.0 mg/L+NAA 1.0 mg/L,with the rooting rate of 82.8%.At last,a rapid propagation technology was developed for common buckwheat plants.