复方虎杖高效液相色谱指纹图谱的研究

详细信息

Study on the Fingerprint Chromatogram of the Compound Giant Knotweed Rhizome by High Performance Liquid Chromatography

College of Chemistry and Engineering, Wuhan University of Technology, Wuhan 430070, China

摘要: 采用高效液相色谱法,建立了复方虎杖指纹图谱的分析方法。色谱条件为:ZORBAX C₁₈(150mm×4.6mm,5μm),检测波长310nm,柱温:25℃,流动相:甲醇(0～85%)∶5%乙酸溶液(100%～15%),梯度洗脱70min。通过分析,确立了复方虎杖指纹图谱的22个共有峰。10批样不同产地的药材指纹图谱具有较好的相似度。该方法准确可靠,重复性好,可用于复方虎杖药材及其制剂的质量控制。
- 虎杖 /
- 高效液相色谱 /
- 指纹图谱
Abstract: The fingerprint chromatogram of the compound giant knotweed rhizome was established by using HPLC,and the sample was separated on a ZORBAX C₁₈(150 mm×4.6 mm,5 μm)colum with the mobile phase of methanol(0-85%)and 5% acetic acid(100%-15%),and apply the gradient elution for 70 minutes.The detection wavelength was 280 nm,and the column temperature was set up at 25℃.By analyzing the fingerprint chromatogram,22 characteristic peaks were selected,and the fingerprint spectrum of 10 places of medicinal substances has high similarity.The HPLC method for fingerprint chromatogram showed high precision and excellent reproducibility,so it can be used in the quality control of the compound giant knotweed rhizome.
- Giant knotweed rhizome /
- HPLC /
- Fingerprint chromatogram