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金属离子抗性衣藻品系的分离筛选及其鉴定

Isolation and Identification of Metal Resistance Green Alga

    摘要
  • 摘要: 从乌鲁木齐南山土壤中分离得到62株绿藻，利用印迹法筛选对Cu2+、Fe3+、Zn2+、Co2+4种金属离子有抗性的藻株。结果发现XJU3、XJU28和XJU36对01 mmol•L-1 Co2+有抗性；XJU28对1 mmol•L-1 Zn2+和 Fe3+有抗性；而XJU36仅对005 mmol•L-1 Cu2+有抗性。利用形态学特征和rDNA转录单元内间隔区（ITS1和TIS2，包括58S）序列对3株绿藻进行了分类学鉴定。依据形态特征，初步判断3株绿藻可能属于衣藻属Chlamydomonas）。利用ITS（包括58S）序列构建系统进化树分析，结果表明，XJU3、XJU28与Chlamydomonas zebra的关系较近，XJU36与Chlamydomonas petasua的关系较近。

     

    Abstract: A total of 62 green alga strains were isolated from the soils of Nanshan Mountain,Xinjiang.Used the blot method to characterize these metal resistance,the results indicated that XJU3、XJU28 and XJU36 have resistance to 01 mmol•L-1Co2+;XJU28 has resistance to 1 mmol•L-1Zn2+ and Fe3+，XJU36 has resistance to 005 mmol•L-1Cu2+.Taxonomic evaluation of the three strains were investigated based on the morphology and the internal transcribed spacer (ITS) regions(including the 58S).Based on morphological characteristics,the three strains were likely to Chlamydomonas.Phylogenetic reconstruction with the Neighborjoining (NJ) method using sequences of ITS(including the 58S) indicated that XJU3 and XJU28 are closed to Chlamydomonas zebra.XJU36 is closed to Chlamydomonas petasua.

     

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