Abstract: The influences of methanol/water solution, extracting time, cultivar/genotype, and culturing days on extraction of total polyphenols in citrus embryogenic calli were studied. The results showed that the highest production of total polyphenols was obtained when citrus calli were homogenized with 40% methanol and incubated at 55℃ for 30 min. Total polyphenol content was cultivar/genotype dependent and highest in ’Anliucheng’ sweet orange compared with that in ’ Jincheng’, ’ Succari’ sweet orange, ’ Ponkan’ tangerine, ’ Murcutt’ hybrid, and ’ Red Marsh’ grapefruit. In addition, total polyphenol content was changed with culturing days, and the highest production of total polyphenols was obtained in citrus calli 40 d after culturing.