Abstract: The fruits of the cultivated Akebia trifoliata and the mice(20-30 g) of species ’Kunming’ were taken as experimental materials.The acute toxicity of pericarp,flesh and seed on these mice were tested respectively.The results indicated that the pericarp and flesh of Akebia trifoliata had no toxicity on these mice.The maximum tolerant dosage for the filtered percarp mixture was 50 g/kg to mice,and the maximum tolerant dosage for the flesh was 100 g/kg to mice.However,the diluted suspended mixture of seeds and distilled water at the ratio of 1 ∶1 was toxic to the mice(LD₅₀=12.83 g/kg).Spontaneous activities of most mice reduced and showed somnolent after the suspended seed mixture was given intragastrically for 5 to 10 minutes,and then died within 1 or 2 hours.A few mice showed light convulsions(hind stretched backwards) before death.Their respiration stopped first and the heart-throb ended subsequently.The toxic symptom of the survived mice continued for 3 or 4 hours and the mice could recover to normal diet and action later.