Effect of Three Growth Regulators on Spore Germination,Protonema Development and Bud Differentiation in Entodon challengeri
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摘要: 研究了3种植物生长调节剂苯基噻二唑基脲(TDZ)、6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)对密叶绢藓[Entodon challengeri(Paris)Cardot]孢子萌发、原丝体发育及芽体发生的影响,并对整个发育过程进行了显微观察和照相,结果表明:(1)3种植物生长调节剂对密叶绢藓孢子萌发影响不显著;(2)在原丝体发育阶段,1.0 mg/LNAA对原丝体初期的发育促进效果显著,0.4 mg/L TDZ对原丝体发育中期分枝的形成促进效果显著,6-BA处理效果不显著;(3)3种植物生长调节剂单独处理均促进芽体的发生,但0.4 mg/L TDZ效果最佳。而1.5 mg/L 6-BA+TDZ组合处理效果更加显著;(4)芽体的发生数量与芽体的长势无正相关性。Abstract: Based on the tissue culture,the effect of N-phenyl-N’-1,2,3-thiadiazol-5-ylurea(TDZ),6-benzyla minoprine(6-BA) and 1-Naphthylacetic acid(NAA) on spore germination,protonema development and bud differentiation in Entodon challengeri was investigated in this paper.The whole process was observed and taken pictures.The results show that TDZ,6-BA and NAA have no significant effect on the spore germination.During the process of protonema development,1.0 mg/L NAA can promote the primary development of protonema significantly,and 0.4 mg/L TDZ promotes branches formation in intermediate stage of the protonema development.The number of bud increases with the concentrations augmentation of the three growth regulators,but it is better under 0.4 mg/L TDZ.And it is the best effect when 1.5 mg/L 6-BA and TDZ is mixed.The bud quantity isn’t correlated directly with its growing trend.
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Keywords:
- Entodon challengeri /
- TDZ /
- 6-BA /
- NAA /
- Spore germination /
- Protonema development /
- Bud differentiation
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