雌雄栝楼的组织培养研究

郭晓亮; 王沫; 舒少华

雌雄栝楼的组织培养研究

Tissue Culture of Male and Female Trichosanthes kirilowii Maxim

摘要

摘要: 对雌雄栝楼(Trichosanthes kirilowiiMaxim)分别进行组织培养的研究结果表明:在雌雄栝楼组织培养过程中,愈伤组织的诱导和分化适宜的培养基均存在性别差异。愈伤组织诱导的适宜培养基分别是,雌性栝楼培养基为MS+NAA0.5 mg/L+IBA0.5 mg/L+6-BA1.5 mg/L;雄性栝楼培养基为MS+NAA0.1 mg/L+IBA0.5 mg/L+6-BA0.5 mg/L和MS+NAA0.1 mg/L+IBA1.0 mg/L+6-BA1.0 mg/L。愈伤组织分化的适宜培养基分别是,雌性栝楼培养基为MS+NAA0.1 mg/L+IBA0.4 mg/L+6-BA1.2 mg/L;雄性栝楼培养基为MS+NAA 0.3 mg/L+IBA0.2 mg/L+6-BA0.8 mg/L;雌雄栝楼无根苗生根的适宜培养基均为MS+NAA0.1 mg/L。

Abstract: The results of tissue culture of male and female Trichosanthes kirilowii Maxim showed that there is difference of phytohormone concentration between male and female T.kirilowii.The suitable medium for the callus induction of female T.kirilowii is the medium MS+NAA 0.5 mg/L+IBA 0.5 mg/L+6-BA 1.5 mg/L.The suitable medium for the callus induction of male T.kirilowii is the medium MS+NAA 0.1 mg/L+IBA 0.5 mg/L+6-BA 0.5 m/L and MS+NAA 0.1 mg/L+IBA 1.0 mg/L+6-BA 1.0 mg/L.The suitable medium for the callus differentiation of female T.kirilowii is the medium MS+NAA 0.1 mg/L+IBA 0.4 mg/L+6-BA 1.2 mg/L.The suitable medium for the callus differentiation to male T.kirilowii is the medium MS+NAA 0.3 mg/L+IBA 0.2 mg/L+6-BA 0.8 mg/L.The suitable medium for rooting to male and female T.kirilowii free seedlings are both MS+NAA 0.1 mg/L.

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