Abstract: Using A-PAGE,genetic polymorphism of hordein was analyzed among 181 wild relatives of barley from 24 different counties in Tibet,including 47 six-rowed wild relatives of barley and 134 two-rowed wild forms.Three barleys that came from Qinghai Province of China,Europe and Israel respectively were used as controls.The hordein patterns showed a very large variation.60 types of hordein patterns were obtained from the tested materials and 38 materials had their unique patterns respectively. The results revealed that 184 materials were clustered into 3 major groups.One major group had only two materials.Two types of hordein patterns that were represented by ZYM0019 and ZYM1488 were widespread and abundant in Tibet,and about 29.3 percent of the tested materials had these patterns.The findings suggested a correlation between hodein patterns and geographic environment.Furthermore,the origin of cultivated barley was discussed.