Abstract: Microsporogenesis of Hong Cai-tai’s Polima cytoplasmic male-sterile line(Polima CMS),Hong Cai-tai’s Ogura cytoplasmic male-sterile line(Ogura CMS)and their maintainers were investigated cytologically by using paraffin section with light microscopy systemly.The observation showed that the anther development of Hong Cai-tai’s Polima male-sterile line was blocked without anther at the stage of archesporial cell.The few pollen and no anther cell was observed without forming the tapetum and the middle layer;while the anther development of Hong Cai-tai’s Ogura male-sterile line was blocked without anther at the stage of microspore mother cell &tetrads of microspores,observing the tapetum cell abnormal and tetrads of microspores being extruded.In the end the tapetum cell and tetrads of microspores disaggregated.