一种紫菜多糖的制备及对淋巴细胞生长的影响
Preparation of Polysaccharide from Porphyra yezoensis Ueda and Its Effects on Growth of Lymphocytes
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摘要: 用DEAE-纤维素和SephadexG-200柱层析法分离纯化条斑紫菜的热水提取物,从中得到多糖PY2,并测出其分子量为2.0×104。用紫外和红外光谱对PY2的性质进行了鉴定。进一步测定了PY2对体外培养的小鼠骨髓细胞及淋巴细胞生长的影响。结果表明,PY2是一种少见的紫菜多糖,它不含有大多数紫菜多糖具有的3,6-内醚-半乳糖和硫酸基,它对小鼠脾脏淋巴细胞、胸腺淋巴细胞以及混合淋巴细胞的增殖有一定的抑制作用,而对骨髓细胞的增殖没有明显的影响。Abstract: The purified polysaccharide PY2 was obtained by DEAE-cellulose and Sephadex G-200 column chromatography from Porphyra yezoensis . The average molecular weight was determined to be 2.0×104 by using column chromatography. PY2 was also identified by UVand IRspectra. The results showed that PY2 is a kind of infrequent polysaccharide from Porphyra ,which could inhibit the proliferation of murine spleen cells, thymus cells and mixed lymphocyte reaction. There were no distinct effects of PY2 on bone marrow cells.
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