Abstract: The phylogenetic relationships of three species of Glycyrrhiza (G.uralensis,G.infleta,G.glebra) were analyzed by RAPD.Nine random primers of high stable quality were screened from 50 primers.The cluster analysis results of 961 amplified DNA fragments were indicated as follows:the average genetic distance among the fifteen groups of Glycyrrhiza was 0.41,of which the max genetic distance was 0.63 while the min 0.26.The results show that there is some difference between RAPD cluster analysis and the original species.