Abstract:
Polysaccharide of branches,leaves,and seeds of
Elaeagnus umbellata Thunb.were extracted with hot water.Protein was excluded using the savage method,and then concentrated and precipitated with 80% ethanol to obtain the polysaccharide.Antioxidant activity of polysaccharide was evaluated by the efficiency of scavenging the hydroxyl radical and superoxide anion radical and reductive potential,with ascorbic acid used as a positive control.Results showed that the polysaccharide with three parts was effective in removing hydroxyl radical,superoxide free radicals,the scavenging activity on hydroxyl radical from the highest to the lowest was seeds>leaves>branches>Vc,while superoxide removal activity from the highest to the lowest was seeds>Vc>leaves>branches.All of polysaccharides possessed strong reducing power,but the effect was not as Thiourea.