Comparison of the Approaches for Full-length cDNA Enrichment
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Abstract: Full-length complementary DNAs (cDNA) are crucial for the functional annotation of genes. Several novel experimental approaches for selective enrichment of full-length cDNAs are outlined in parallels. Some kinds of reverse transcriptases and high fidelity PCR enzymes are introduced for promising high efficiency of accurate full-length cDNA synthesis. The temperature profiles of the reverse transcription and PCR are also discussed.
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Keywords:
- Full-length cDNA /
- mRNA /
- 5'-CAP /
- High-fidelity PCR /
- Comparison
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