Abstract: Crossing experiments of 68 combinations within and between genera of Magnoliaceae were made in the Fairy Lake Botanical Garden based on the conventional methods of hybridization. Results revealed that the pollen vigor was various among different species. The low rate of pollen germination was not an important factor responsible for the low fruit set since owning a large number of pollens and having a high fruit set after manual crossing. The cross-compatibility commonly existed in the same genus of Magnoliaceae, except genus Magnolia (between subgenus Magnolia and Yulania). Furthermore, reproductive isolation between some species of Michelia was found. Subgenus Yulania and genus Michelia had a very close relationship, for their crossing being highly compatible and the hybrids being vigorous. Since it had reproductive isolation with Michelia, genus Tsoongiodendron should be accepted.