湖北省优质杂交稻品种贮藏蛋白的比较研究

何莹; 邵锦震; 余夏夏; 王前和; 朱伯华; 丁毅

湖北省优质杂交稻品种贮藏蛋白的比较研究

Study on Storage Protein of High Quality Hybrid Rice in Hubei Province

摘要

摘要: 研究选取水稻品种共20个,其中包括湖北省近两年审定的优质杂交水稻品种10个(‘鄂早17’、‘鄂早18’、‘两优1193’、‘武香880’、‘岳优26’、‘宜优99’、‘协优96’、‘鄂晚12’、‘鄂中5号’、‘两优277’)以及7个普通杂交水稻(‘G98-202’、‘578’、‘两优637’、‘宜优22’、‘3685’、‘3089’、‘加育948’)和国家审定的优质水稻品种3个(‘嘉育948’、‘两优932’、‘舟903’),采用不连续的SDS-PAGE电泳分析种子贮藏蛋白,结果在谷蛋白中主要分离出57 kD的蛋白前体、37～39 kD的酸性亚基和22～23 kD的碱性亚基。同时,选取品种‘3089’、‘嘉育948’、‘537’、‘舟903’和‘两优932’,利用高效液相色谱法分析了种子贮藏蛋白中的谷蛋白亚基的含量。结果表明,水稻谷蛋白亚基含量的多少可以作为评价品种营养品质优劣的参考依据之一。

Abstract: Twenty cultivars of hybrid rice were chosen in this study,of which including ten cultivars of high quality hybrid rice(‘Ezao 17’,‘Ezao 18’,‘Liangyou 1193’,‘Wuxiang 880’,‘Yueyou 26’,‘Yiyou 99’,‘Xieyou 96’,‘Ewan 12’,‘Ezhong 5’,‘Liangyou 277’) identified by Hubei Province in two years and seven ones of general hybrid rice(‘G98-202’,‘578’,‘Liangyou 637’,‘Yiyou 22’,‘3685’,‘3089’,‘JY 948’),and three kinds of high quality hybrid rice which have passed the national identification(’Jiayu 948’,’Liangyou 932’,’Zhou 903’) as control.Using discontinuous SDS-PAGE,the storage glutelin of rice seeds were separated into a 57 kD precursor,a 37-39 kD acidic subunit and a 22-23 kD basic subunit.Five kinds of hybrid rice ‘3089’,‘Jiayu 948’,‘537’,‘Zhou 903’ and ‘Liangyou 932’ were chosen to analyze the content of the glutelin subunit by HPLC(high performance liquid chromatography).The results showed that the content of the glutelin subunit could be regarded as a standard of distinguishing between high and low quality rice.

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