发菜(Nostoc flagelliforme)培养条件的研究
Studies on the Cultivation of Nostoc flagelliforme
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摘要: 研究了光照强度、日供水次数、CO2浓度和培养基成分对发菜生长的影响。结果显示,中度光强(114μmol.m-2.s-1)下发菜生长最快;发菜的生长基本同供水次数成正相关系;CO2浓度的升高并没有显著促进发菜生长,低光条件下(57μmol.m-2.s-1),高浓度的CO2(2800μL/L)抑制了发菜的生长;用BG110培养的发菜生物量的增长显著高于用BG110培养的;BG11培养基中K+和CO32-的缺失并没有显著影响发菜的生长。Abstract: The effects of light intensities, watering times per day, CO2 concentrations and culture medium on the growth of Nostoc flagelliforme were studied. N. flagelliforme grew best under middle light intensity (114 μmol·m-2·s-1). The growth of N. flagelliforme was positively rela ted with watering times per day. Higher CO2 concentration(2800 μL/ L) did not significantly accelerate the growth of N. flagelliforme. Under the light intensity of 57 μmol·m-2·s-1, the growth of N. flagelliforme was significantly inhibited by higher CO22- concentrations. N. flagelliforme grew better when cultured with BG11 medium than that with BG110 medium. The lack of K+ and CO32- in BG11 medium did not significantly affect the growth of N. flagelliforme in the experiment.