中华结缕草(<I>Zoysia sinica</I> Hance)组织培养和再生植株研究

中华结缕草(Zoysia sinica Hance)组织培养和再生植株研究

详细信息

1.
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan430074, China
2.
Research Institute of Forestry, Chinese Academy of forestry, Beijing100091, China
3.
Beijing Forestry University, Beijing 100083, China

摘要: 以中华结缕草(Zoysiasinica Hance)成熟种子为外植体在附加2.5mg/L2,4-D、0.25mg/L6-BA和1～2mg/LVB₁的改良MS培养基(MS_m)上愈伤组织的诱导率最高为43.0%。愈伤组织的最佳继代培养基为MSm附加0.1mg/L6-BA和2.0mg/L2,4-D。在无生长调节物质的MS培养基(MS₀)上,外观呈白色到淡黄色、含有密实颗粒的愈伤组织再生率为30%～60%。
- 中华结缕草 /
- 成熟胚 /
- 愈伤组织 /
- 植株再生
Abstract: Mature seeds were used as explant to study the callus induction, subculture, and plant regeneration of Zoysia sinica Hance. The results showed that the maximum rate of callus induction was 43.0% and occurred on the modified MS medium (MS_m) supplemented with 2.5 mg/L 2,4-D, 0.25 mg/L 6-BA, and 1-2 mg/L thiamine. The optimum subculture medium was MS_m containing 0.1 mg/L 6-BA and 2.0 mg/L 2,4-D. 30%-60% calli type of white to yellowish could regenerate plants on MS medium free of growth regulators.
- Zoysia sinica Hance /
- Mature embryo /
- Callus /
- Plant regeneration