Abstract: The gene Dad-1 is a well-conserved programmed cell death (PCD) suppressor gene in animals and plants.In this study,the single copy rice gene Dad-1 was used as probe to investigate its homology and physical localization in Coix lacryma-jobi genome.The results of Southern blotting suggested that there were sequences homologous to rice Dad-1 gene in C.lacryma-jobi genome.Further,the sequences were mapped on the short arm of chromosome 9 in C.lacryma-jobi by fluorescence in situ hybridization (FISH).The percent distance from the signal site to centromere was 67.44±1.45 .