Abstract: An addition line of wheat-rye was selected and is named "Addition Line No.1" tentatively. Its agricultural characters are stable. It possesses high number of grains per spikelet and good plumpness. Ears and grains are rather big.The protein content of "Addition Line No.1" is 17.00-18.29%, and lysine content up to 0.49-0.54%.The meiosis in PMC shows complete pairing, with 22Ⅱ at diakinesis, and normal disjunction 22-22 at anaphase Ⅰ,and 2n=44 in root tip cells was observed at metaphase. The number of chromosomes described above is stable in about 90% of cells. The result of karyotype analysis shows that 2 chromosomes of the addition line are longer than the chromosomes of wheat.The heterochromatin was observed on the tip of 2 chromosomes, using C-banding. The PAGE analysis of esterase isozymes shows that it has a rye-markband. So, a disomic alien addition line of wheat-rye has been obtained.