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浮丝藻生活史及其亚显微结构特征的研究

STUDIES ON THE LIFE CYCLE AND ULTRASTRUCTURE CHARACTERISTICS OF PLANKTONEMA

  • 摘要: 通过观察浮丝藻(Planktonema)的生活史、显微和亚显微结构、细胞分裂方式,系统地研究了其藻丝体的生物学特性。浮丝藻为单列细胞不分枝的丝状体,细胞圆柱形或椭圆形,细胞之间主要靠筒状的纤维素细胞壁相互连接。藻细胞中含叶绿素a、b和β-胡萝卜素。电镜下,细胞壁明显分层,绝大多数是2~3层;色素体片状、周生,通常2至多条类囊体重叠;1~2个蛋白核在色素体体上,蛋白核外常有淀粉鞘,有时1至多条类囊体重叠;1~2个蛋白核在色素体上,蛋白核外常有淀粉鞘,有时1至多条类囊体侵入蛋白核内;1~2个线粒体位于色素体旁,嵴不分枝;高尔基复合体靠近细胞周边,分泌许多潴泡;细胞核1个,球形或不规则方形,常靠近细胞的一端;光镜下细胞两端的折光亮点在电镜下是1至多个泡囊,泡囊内通常含些被染色很深的物质。浮丝藻的形态学、细胞分裂和生活史观察结果都证明,浮丝藻的细胞在整个生活史中没有营养分裂,细胞分裂时母细胞产生两个有独立、完整细胞壁的似亲孢子,一系列似亲孢子通过存留的、不连续的筒状或杯状母细胞壁连接成形状上象丝状体的结构。因此,藻丝体形成和成长的过程是一个无性繁殖过程,这样的藻丝体本质上是一种假丝体。

     

    Abstract: Biological characteristics of Planktonema were systematically studied by observing its life cycle,microstructure,ultrastrutture and cell division. Planktonema is unbranched filament in a row of cell. The cells are cylindric or elliposoid,mainly joined by cylindric cellulose cell walls and the cells contain chlorophyll a,b and β-carotene. Under transmission electron microscope. the cell walls clearly stratify and mostly have 2~3 layers. The chloroplast is lamellar, parietal and usually two or several thylakoids overlap;there are 1~2 pyrenoids in chloroplast and the pyrenoids have envelopes of starch grains,sometimes one or several thylakoids penetrating the pyrenoid. 1 ~2 mitochondrions stand by the chloroplast, and the ridges do not branch. Golgi complex sits near the cell circumference and excretes many vesicles. One nucleus is irregular square or globular, close to one pole of the cell. One or several vacuoles,which present bright spots at both poles of the cell under light microscope,usually contain some material dyed deeply. The studies on the morphology, the cell division and the life cycle demostrate the cells have not desmochisis and by the cell division the mother cell produces two autospores with their own complete cell walls. Aseries of autospores are linked to become the filamentous forms by the remaining, discontinuous cylinder-shaped or cup-shaped mother cell wall, and the formation and development of the filaments is fulfilled by asxeual reproduction, therefore, this kind of filaments naturally are the pseudo-filaments.

     

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