Abstract: Three transgenic wheat lines have been evaluated under field conditions during 2 years continuously, together with 4 non-transgenic lines of wheat. Two of the non-transgenic lines are donor plants of the transgenic lines and the other two are local region cultivars used as control materials. The plant growth and development of all transgenic lines were normal under the conditions tested. But their morphological and major agronomic traits were different from the donor plants, especially when comparing with 2 local cultivars. In the yield and 1000 grains weight, there was difference of major agronomic traits between transgenic lines and its donor lines. Results of the histochemical and SDS-PAGE analysis showed that the target genes were stably inherited.