6-苄基腺嘌呤对平菇和香菇菌丝体4种胞外酶活性的影响

The Effect of 6-Benzyl Aminopurine on the Activities of Four Extracellular Enzymes in Plurotus ostreatus and Lentinula edodes Mycelium

摘要: 在马铃薯液体培养基中,通过添加不同剂量的6-苄基腺嘌呤(6-BA),测定了平菇和香菇菌丝体4种胞外酶活性的变化.结果表明,低浓度的外源6-BA对平菇、香菇菌丝体4种胞外酶活性都有促进作用,高浓度时则逐渐抑制酶活性.其中,培养液浓度为1.5mg/100mL时,它们的胞外淀粉酶(Amy)、羧甲基纤维素酶(CMC)、过氧化氢酶(CAT)的活性最高;而它们的胞外蛋白酶的最适浓度为0.5mg/100mL.

Abstract: Four extracellular enzyme activities in Pleurotus ostreatus and Lentinula edodes mycelium were determined by adding to different dosage additional 6-benzyl aminopurine (6-BA) in potato liquid medium. The results showed that low concentration of 6-BA promoted their activities and high concentration inhibited their activities. For Pleurotus ostreatus and Lentinula edodes, the optimum concentration of 6-BA to activate their extracellular amylase, carboxymenthel cellulase and catalase was the same which was 1.5 mg/100 mLculture solution. But, the optimum concentration for their extracellular protease was 0.5 mg/100 mLculture solution.