尼日利亚螫毛果(Cnestis ferruginca)中一种具有清除自由基的活性化合物的分离鉴定
Isolation and Identification of a Free Radical Scavenger from Cnestis ferruginca
-
摘要: 用二苯基苦基苯肼自由基薄层实验法(DPPH-TLC-ASSAY),从尼日利亚螫毛果(Cnestisferruginca)植物的甲醇提取物中发现了一种具有很强的清除自由基活性的物质;用柱色谱及反相制备柱色谱(RP-LPPLC),制备出了该活性物质的纯品;用质谱(HRESI-MS)、核磁共振氢谱(1H)、碳谱(13C、DEPT)、紫外光谱及化学方法对该物质进行了结构鉴定,鉴定结果为对苯酚基-6-O-反式咖啡酸基-β-D-吡喃葡萄糖甙.Abstract: By means of 1,1-dipheny-2-picylhydrazyl (DPPH) TLC-assay, a strong free radical scavenger was detected from a MeOH extract of the leaves of Cnestis ferruginca. The free radical scavenger was isolated by reversed-phase preparative chromatography and liquid chromatography, and identificated as p-hydroxyphenyl-6-O-trans-caffeoyl-β-D-glucopyranoside by means of HR ESI-MS, NMR (1H, 13C,DEPT), UVand chamical methods.The bioactivities of this compound were also discussed.
下载: