Abstract: The authers introduced a simple and convenient procedure of sifting and separating aescin with resins: Firstly we used static state and batch adsorption method to compare. Secondly we affirmed it with dynamic state column chromatography. The effect of separation aescin with five resins (D101、D4020、H103 NKA-9201×4) and silica Gel G was checked. The result indicated that NKA-9 resin has capacity of good adsorption、separative adsorption and specificity to aesein.