Abstract: Several methods were used to extract the flavonoids from the leaves of Sargentodoxa cuneata while concentration of flavonoids in the extraction was determined by different methods. The suitable quantitative methods used in the ecological analysis of flavonids was that using ultraviolet and 70% ethanol was the best reagent for extraction. Heating was not necessary for extraction. The content of flavonoids from different organs was leafblade, annual twig, leafstalk and perennial stem in turn.