Abstract: Using the LKB-2277 bioactivity monitor,five iso-thermal curves of anthers including four rice CMS(cytoplasm male sterile) lines Purple rice A,Zhenshan 97A, Honglian Yuetai Aand Maxie Aand one maintainer Purple rice Bwere obtained at 37℃.The results indicated that every curve had its character which was directly correlative to the degree of pollen abortion and consistent with the cytologic analysis, eg.,the numerical value of Purple rice Aanther's curve is minimum and the anther's metabolize activity is the weakest correspondingly,and so are the others.This is a new method that can be used in qualitative and quantitative analysis of rice male sterility.