藻红蓝蛋白α-亚基脱辅基蛋白与藻蓝胆素的重组

1.
武汉大学生命科学学院, 武汉 430072
2.
华中科技大学环境科学与工程学院, 武汉 430074
3.
BotanicInstitute, UniversityMunich, MenzingerStr. 67, D-80638Muenchen, Germany

详细信息

Reconstitution of Phycocyanobilin with the Overexpreesed Apo-α-phycoerythrocyanin

1.
College of Life Sciences, Wuhan University, Wuhan 430072, China
2.
College of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
3.
Botanic Institute, University Munich, Menzinger Str. 67,D-280638 Muenchen, Germany

摘要: 利用在大肠杆菌中表达的藻红蓝蛋白α-亚基脱辅基蛋白与藻蓝胆素PCB重组,吸收光谱、荧光光谱和高效可逆光化学性质分析表明,藻红蓝蛋白α-亚基脱辅基蛋白与藻蓝胆素直接重组,生成的胆素蛋白中辅基色素仍为藻蓝胆素;而藻红蓝蛋白α-亚基脱辅基蛋白与藻蓝胆素在藻红蓝蛋白α-亚基重组酶(pecE和pecF基因的表达产物)催化下重组,生成的胆素蛋白中辅基色素转变为藻紫胆素,并具有高效可逆光化学特性。
- 藻红蓝蛋白α-亚基 /
- 重组 /
- 高效可逆光化学特性
Abstract: Using the expressed and renatured apo-α-phycoerythrocyanin to be reconstituted with phycocyanobilin, the prosthetic group in the reconstitued phycobiliproteins were analyzed with absorption spectra. When apo-α-phycoerythrocyanin was reconstituted with phycocyanobilin directly, the prosthetic group in the produced phycobiliprotein is still phycocyanobilin. However, when apo-α-phycoerythrocyanin was reconstituted with phycocyanobilin under catalysis of α-phycoerythrocyanin lyase, the prosthetic group in the produced phycobiliprotein is phycoviolobilin, which possessed the highly reversible photochemistry property.
- phycoerythrocyanin /
- Reconstitution /
- Highly reversible photochemistry