Abstract: Salt-tolerant callus was selected from immature embryo and young inflorescence segments of wheat (Triticum aestivum) which seeds were carried by spacecraft,and the salt tolerant stability of their progenies were studied on physiological and biochemical characterization.The results showed that:(1) The relative content of free proline in salt-selected wheat plantlet’s progencies was higher than that in unselected ones at higher NaCl concentration soils;(2) The salt-selected lines had higher K⁺/Na⁺ ratio;(3) Four additional specific electrophoretic bands of gliadin (b2,b3,b5 and b7) were detected and one band (b8) disappeared in F₄ seeds from salt-selected lines;(4) 26 electrophoretic bands of soluble protein were observed in F₄ plants from salt-selected lines,whereas only 23 bands in control lines.Of which 98 kD,75 kD,52 kD,49 kD and (32 kD) bands were specific in salt-tolerant line,38 kD and 35 kD protein bands only appeared in (control) lines.