摘要:
以卫星搭载小麦种子为原始材料,利用其幼穗、幼胚诱导的愈伤组织进行耐盐突变体的筛选,对耐盐愈伤组织再生植株后代进行耐盐稳定性生理生化特性分析。结果表明:(1)耐盐系后代在土壤高盐浓度条件下,游离脯氨酸含量稳定增加,且高于对照系;(2)耐盐系再生植株后代保持较高的K+/Na+比;(3)与对照相比,种子醇溶蛋白电泳带谱中的b2,b3,b5,b7带为耐盐系所特有,b8带消失;(4)耐盐系再生植株后代可溶蛋白电泳带为26条,而对照系为23条蛋白带。其中98kD、75kD、52kD、49kD和32kD为耐盐系的特有蛋白带。而38kD和35kD蛋白带为对照系所特有。
Abstract:
Salt-tolerant callus was selected from immature embryo and young inflorescence segments of wheat (Triticum aestivum) which seeds were carried by spacecraft,and the salt tolerant stability of their progenies were studied on physiological and biochemical characterization.The results showed that:(1) The relative content of free proline in salt-selected wheat plantlet’s progencies was higher than that in unselected ones at higher NaCl concentration soils;(2) The salt-selected lines had higher K+/Na+ ratio;(3) Four additional specific electrophoretic bands of gliadin (b2,b3,b5 and b7) were detected and one band (b8) disappeared in F4 seeds from salt-selected lines;(4) 26 electrophoretic bands of soluble protein were observed in F4 plants from salt-selected lines,whereas only 23 bands in control lines.Of which 98 kD,75 kD,52 kD,49 kD and (32 kD) bands were specific in salt-tolerant line,38 kD and 35 kD protein bands only appeared in (control) lines.
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