红莲型细胞质雄性不育水稻线粒体DNA的AP-PCR分析

详细信息

AP-PCR Analysis for Mitochondria DNA of CMS Rice Honglian Type

The Key Laboratory of MOE for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China

摘要: 为了研究红莲型细胞质雄性不育与线粒体基因组的关系,以水稻红莲型粤泰细胞质雄性不育系A和保持系B及杂种一代F₁为材料,应用AP-PCR分析,用10个单引物对其线粒体DNA进行扩增。实验结果表明,不同的引物在3种材料间均有不同程度的差异,为红莲型细胞质雄性不育分子机理的研究提供了线索;此外,在引物6F₁的扩增图谱中找到一条在YTA和F₁中特异的带TAF_6F2,Sounthern分析TAF_6F2不育胞质的特异性,可能与红莲型水稻细胞质雄性不育性状的形成有关。
- 细胞质雄性不育 /
- 线粒体DNA /
- AP-PCR /
- 水稻
Abstract: An economic and efficient DNApolymorphism assay,AP-PCR,was adopted to Honglian type rice mitochondrial DNA.The result indicated obvious differences in the mt-DNA of CMS line,maintain line and its hybrid,which could further the research of CMS mechanism.Through the comparation of fingerprints among the materials,a CMS line specific mt-DNAb and TAF_6F2 was isolated.Southern Blot showed it only existed in the mt-DNA of CMS line and the first generation hybrid.This band may be relative to the formation of CMS in Honglain type rice.
- Cytoplasmic male sterility /
- Mitochondrial DNA /
- AP-PCR /
- Rice