Abstract: Oryza officinalis is an important resistance resource for improvement of cultivated rice.In this study,cultivated rice BAC clone 14E16 and RFLP marker RZ262 which linked to RTSV,Glh were adopted for mapping in O.officinalis.The FISHresults showed 14E16 and RZ262 were located at 4Sin O.officinalis,the percentage distance from the centromere to the hybridization sites were 74.86±3.72和73.98±4.44,the detection rates were 7.6% and 45.1% respectively.The results obtained from the BAC and plasmid clones RZ262 of cultivated rice and O.officinalis were the same,it is suggested that the marker,RZ262,of cultivated rice and O.officinalis be in the same BAC clone,the homologous sequences of RTSV and Glh in O.officinalis were positions that signals exited on the 4S.These results will provide the base for utilizations of resistance genes in wild rice.