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用RFLP标记剖析水稻穗颈维管束及穗部性状的遗传基础

Genetic Dissection of Vascular Bundle Systems in Peduncle and Panicle Characters in Rice (Oryza sativa L.) by Means of RFLP Markers

  • 摘要: 采用籼、粳亚种间杂种F1<(圭630×02428)花药培养获得的DH群体,对水稻穗颈大、小维管束数和倒数第2节间大、小维管束数等4个维管束性状,以及一、二次枝梗数,每穗颖花数3个穗部性状进行QTL分析,共检测到16个QTLs,其中有7个QTLs的加性效应较大,单个QTL的贡献率在20%以上。发现有4个QTLs成簇分布于第1染色体从RZ776到C11的大约35cM的区段上,来源于亲本"圭630"的这一染色体区段对穗颈大维管束、第2节间大维管束、第2节间小维管束和二次枝梗数4个性状的表达均具有增效作用。还讨论了利用分子标记辅助选择聚合增效QTLs、实现穗颈维管束性状遗传改良的策略。

     

    Abstract: A doubled-haploid rice population,consisting of 81 DH lines derived from the F1 hybrid of a cross between indica cultivar and japonica cultivar,was used to map quantitative trait loci(QTL) controlling numbers of vascular bundles in the peduncle and the top second internode,primary and secondary rachis branches,and spikelets per panicle.A total of 16 QTLs were detected.Among them,7 QTLs with larger effects each explained more than 20% of the total variation for the traits concerned.A cluster of 4 QTLs for the traits studied was located to the genomic region of about 35 cM in length flanked by the marker RZ776 and C11 on chromosome 1.This chromosome region from the parent ’Gui630’ contributed to the increasing phenotypic values of the 4 traits,viz.numbers of large vascular bundles in peduncle and top second internode,small vascular bundles in top second internode,and secondary rachis branches.The present paper also discussed the strategy for improving of vascular bundle system in peduncle via pyramiding favorable QTLs for the traits concerned using marker-assisted selection.

     

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