ISOLATION, PURIFICATION AND CHARACTERIZATION OF POLYSACCHARIDES FROM PRASIOLA YUNNANICA
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摘要: 从云南溪菜中提取的水溶性胞外粗多糖(EPS), 用Sevag法脱蛋白后, 经DEAE-纤维素柱层析和葡聚糖凝胶G-200柱层析可得到纯品。溪菜多糖复合物经碘试验证明含有少量淀粉, BaCl2试验检测出含有SO42-, 琼脂糖凝胶电泳显示含有糖类及蛋白质, 且其组成不均一。用Lowry法测得蛋白质含量为13.54%, 苯酚-硫酸法测得糖含量为78%。经薄层层析和纸层析鉴定, 溪菜多糖复合物的酸水解物中含有大量D-甘露糖(D-Mannose), 少量D-核糖(D-Ribose)及L-鼠李糖(L-Rhamnose), 其含量比为4: 1.5: 1Abstract: The polysaccharides extracted from prasiola yunnanica.by hot water are EPS (extracellular polymeric substances).They are deproteinized by Sevag method for several times and purified by DEAE-cellulose and Sephadex G-200 chromatography column in turn.The crude polysaccharides were proved to have a little starch and some SO42- by iodine indicator and BaCl2 solution respectively.Agarose gel electrophoresis shows the existence of sugar and protein which are inhomoaenous.It has 13.54% of protein essayed by Lowry method, and 78% of sugar by benzene-H2SO4 method. And the rest are ash and water. The components of the crude polysaccharides were identified by paper chromatography and TLCwhich were D-Mannose, D-Ribose and L-Rhamnose with the ratio of 4: 15: 1. D-Ribose in the algal polysaccharides studies is quite rare.
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Keywords:
- Prasiola yunnanica /
- Polysaccharides
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期刊类型引用(2)
1. 杜昊楠，兰国玉，吴志祥，陈伟，杨川. 海南热带雨林土壤细菌组成与多样性分析. 南方农业学报. 2022(03): 840-849 . 
百度学术
2. 杜昊楠，陈伟，兰国玉，吴志祥，杨川. 海南热带雨林土壤真菌时空分布格局. 生态学杂志. 2021(08): 2450-2459 . 百度学术
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